MBS excels at recombinant protein production and advanced chemical modifications including fluorophore attachment, biotinylation, and chemical crosslinking. We specialize in both small-sale (ug – mg scale) and large-scale production (gram- kg scale), with considerable expertise in production scale-up to larger quantities and batch sizes. Highest quality standards are carefully monitored at every stage in the process.
de novo Construction
Typical project workflow (each project will be evaluated individually to meet client needs). Most of our services listed below are also available individually as a fee-for-service.
- Plasmid design/codon optimization (highly recommended)
- Selection of cell strain
- mg scale (shake flask) expression optimization
- includes isolation of soluble and insoluble protein and SDS-PAGE analysis
- 10-130 L fermentation (animal-free available)
- Mechanical Lysis
Small-Scale Protein Production:
In addition to single protein production, Lytic Solutions also has the capacity to handle high-throughput variant gene synthesis and protein production capabilities. If you are having trouble expressing your protein, let us try. We have specialized equipment, tools, and experience to get orders of magnitude higher production than less equipped facilities.
Large Scale Production & Industrial Process Development:
With 10L, 20L, 30L, 75L, and 130L fermentation vessels, Lytic Solutions can handle gram to kilogram protein quantity production capacity. We can help troubleshoot your protein production issues and then produce massive quantities of your recombinant protein.